肺轉(zhuǎn)移瘤血管生成機(jī)制的X光光柵相襯成像

摘要

目的：評(píng)估基于光柵的相襯成像 (GPI) 技術(shù)在不使用造影劑的情況下研究裸 BALB/c 小鼠的腫瘤血管生成機(jī)制的可行性。

方法：將中分化胃癌細(xì)胞株SGC-7901注入裸鼠尾靜脈，建立了人胃癌肺轉(zhuǎn)移模型。將樣品浸入 10% 福爾馬林懸浮液中并在成像前干燥。在上海同步輻射裝置 (SSRF) 的 BL13W 光束線上獲得了基于光柵的 X 射線相位襯度圖像，并與組織切片進(jìn)行了比較。

結(jié)果：在沒有造影劑的情況下，基于光柵的 X 射線相位襯度成像仍能以高空間分辨率區(qū)分轉(zhuǎn)移性腫瘤內(nèi)的血管生成。小至幾十微米的血管顯示出與周圍腫瘤不同的灰度值，這使得它們很容易被識(shí)別。在影像學(xué)研究中描繪的血管與組織病理學(xué)中發(fā)現(xiàn)的血管相似，無(wú)論是大小還是形狀。

結(jié)論：我們的初步研究表明，基于光柵的 X 射線相位襯度成像具有描繪肺轉(zhuǎn)移灶血管生成的潛力。

Grating-Based Phase-Contrast Imaging of Tumor Angiogenesis in Lung Metastases

Abstract

Purpose: To assess the feasibility of the grating-based phase-contrast imaging (GPI) technique for studying tumor angiogenesis in nude BALB/c mice, without contrast agents.

Methods: We established lung metastatic models of human gastric cancer by injecting the moderately differentiated SGC-7901 gastric cancer cell line into the tail vein of nude mice. Samples were embedded in a 10% formalin suspension and dried before imaging. Grating-based X-ray phase-contrast images were obtained at the BL13W beamline of the Shanghai Synchrotron Radiation Facility (SSRF) and compared with histological sections.

Results: Without contrast agents, grating-based X-ray phase-contrast imaging still differentiated angiogenesis within metastatic tumors with high spatial resolution. Vessels, down to tens of microns, showed gray values that were distinctive from those of the surrounding tumors, which made them easily identifiable. The vessels depicted in the imaging study were similar to those identified on histopathology, both in size and shape.

Conclusions: Our preliminary study demonstrates that grating-based X-ray phase-contrast imaging has the potential to depict angiogenesis in lung metastases.

文章鏈接：Lin, Huimin, et al. "Grating-based phase-contrast imaging of tumor angiogenesis in lung metastases." PloS one 10.3 (2015): e0121438.

文章中所使用的光柵是由德國(guó)Microworks生產(chǎn)定制、生產(chǎn)的（點(diǎn)擊了解詳情）。